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Gene Expression Data
Assay Details
Assay
Reference: J:65401 Sheth B, et al., Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mech Dev. 2000 Oct;97(1-2):93-104
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3044895
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: R40.76 (MABT11)
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Secondary antibody was labeled with either FITC or Alexa 488. Dual labeling confocal microscopy.
Results
Specimen 1A,C,D: embryonic day 2.5 (more )
Note: Compact 8-cell embryo. Dual labeling confocal microscopy with Catna1 as shown in assay: MGI:3044899.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1C , 1D , 1A Expression is within the apicolateral cell contact region.

Specimen 1E,G,H,I: embryonic day 3.0 (more )
Note: 16-cell embryo. Dual labeling confocal microscopy with Catna1 as shown in assay: MGI:3044899.
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 1I , 1G , 1E , 1H Expression is within the apicolateral cell contact region.

Specimen 1J,L,M: embryonic day 3.5 (more )
Note: Early blastocyst. Dual labeling confocal microscopy with Catna1 as shown in assay: MGI:3044899.
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 1L , 1M , 1J Expression is within the apical cell contact region.

Specimen 2A,C,D: embryonic day 3.5 (more )
Note: Early blastocyst. Dual labeling confocal microscopy with Cgn as shown in assay: MGI:3044903.
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2C , 2A , 2D Expression is within the apicolateral cell contact region.

Specimen 2E,G,H: embryonic day 3.5 (more )
Note: Early blastocyst. Dual labeling confocal microscopy with Ocln as shown in assay: MGI:3044902.
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2H , 2E , 2G Expression is within the apicolateral cell contact region.

Specimen 6E,F: embryonic day 2.5 (more )
Note: Compact 8-cell embryo. Dual labeling confocal microscopy with Rab13 as shown in assay: MGI:3044908.
Structure Level Pattern Image Note
TS3: compacted morula Present 6F , 6E Expression is in discontinuous membrane foci along the lateral membrane and the apicolateral margin.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/13/2022
MGI 6.21
The Jackson Laboratory