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Gene Expression Data
Assay Details
Assay
Reference: J:65401 Sheth B, et al., Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mech Dev. 2000 Oct;97(1-2):93-104
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3044895
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: R40.76 (MABT11)
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Secondary antibody was labeled with either FITC or Alexa 488. Dual labeling confocal microscopy.
Results
Specimen 1A,C,D: embryonic day 2.5 (more )
Note: Compact 8-cell embryo. Double labeled: green - Tjp1; red - Ctnna1 (assay MGI:3044899).
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 1C , 1D , 1A Expression is within the apicolateral cell contact region.
Specimen 1E,G,H,I: embryonic day 3.0 (more )
Note: 16-cell embryo. Double labeled: green - Tjp1; red - Ctnna1 (assay MGI:3044899).
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 1I , 1G , 1E , 1H Expression is within the apicolateral cell contact region.
Specimen 1J,L,M: embryonic day 3.5 (more )
Note: Early blastocyst. Double labeled: green - Tjp1; red - Ctnna1 (assay MGI:3044899).
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 1L , 1M , 1J Expression is within the apical cell contact region.
Specimen 2A,C,D: embryonic day 3.5 (more )
Note: Early blastocyst. Double labeled: green - Tjp1; red - Ocln (assay MGI:3044902).
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2C , 2A , 2D Expression is within the apicolateral cell contact region.
Specimen 2E,G,H: embryonic day 3.5 (more )
Note: Early blastocyst. Double labeled: green - Tjp1; red - Cgn (assay MGI:3044903).
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2H , 2E , 2G Expression is within the apicolateral cell contact region.
Specimen 6E,F: embryonic day 2.5 (more )
Note: Compact 8-cell embryo. Double labeled: red - Tjp1; green - Rab13 (assay MGI:3044908).
Structure Level Pattern Image Note
TS3: compacted morula Present 6F , 6E Expression is in discontinuous membrane foci along the lateral membrane and the apicolateral margin.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
MGI 6.23 		The Jackson Laboratory