Probe:	Sorcs2 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Identical results were obtained using probe Sorcs2 cDNA4 (MGI:2677006), containing nucleotides 2580-4038 of AY004316. The authors did not specify which probe was used in the pictured assay.

Structure	Level	Pattern	Image	Note
TS19: lung epithelium	Strong		2A
TS19: neural tube floor plate	Strong		2A
TS19: midbrain floor plate	Strong		2A
TS19: myelencephalon floor plate	Strong		2A
TS19: heart	Present		2A
TS19: pre-cartilage condensation	Present		2A
TS19: somite	Present		2A
TS19: embryo	Present	Regionally restricted	2A	Expression is in connective tissue.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong		2B
TS22: medulla oblongata floor plate	Strong		2B
TS22: midbrain floor plate	Strong		2B
TS22: hindbrain	Strong	Regionally restricted	2B	Expression is in the floor plate.
TS22: cartilage	Present		2B
TS22: skeletal muscle	Present		2B
TS22: embryo	Present	Regionally restricted	2B	Expression is in connective tissue.
TS22: heart	Weak		2B

Structure	Level	Pattern	Image	Note
TS24: cartilage	Present		2C
TS24: lung epithelium	Present		2C
TS24: thalamus	Present		2C
TS24: embryo	Present	Regionally restricted	2C	Expression is in connective tissue.
TS24: skeletal muscle	Weak		2C
TS24: smooth muscle tissue	Weak		2C

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Weak	Regionally restricted	2D	Expression is in the ventral region extending to the ventral horns.
TS17: neural tube floor plate	Strong		2D

Structure	Level	Pattern	Image	Note
TS19: neural tube floor plate	Strong		2E
TS19: future spinal cord	Present	Regionally restricted	2E	Expression is in the ventral spinal cord extending dorsally.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Strong		2F

Structure	Level	Pattern	Image	Note
TS22: spinal cord floor plate	Weak		2G
TS22: spinal cord	Present	Regionally restricted	2G	Expression is in the ventral spinal cord extending dorsally.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon floor plate	Strong		2H

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata floor plate	Strong		2I

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata floor plate	Present		2J
TS22: medulla oblongata basal plate ventricular layer	Present		2J

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2K	Expression is in the dopaminergic region.
TS19: diencephalon	Present	Regionally restricted	2K	Expression is in the thalamus.
TS19: diencephalon lateral wall marginal layer	Present		2K
TS19: telencephalon marginal layer	Present		2K

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present		2L
TS24: cerebral cortex ventricular layer	Present		2L
TS24: diencephalon lateral wall ventricular layer	Present		2L

Structure	Level	Pattern	Image	Note
TS24: brain	Present	Regionally restricted	2M	Strong signals over the ependymal layer and weak signals over the substantia nigra.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	2N	Expression is in the mediodorsal thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	2O	Expression is in the mediodorsal thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS24: head mesenchyme	Present	Regionally restricted	3A	Expression is in facial mesenchyme.
TS24: olfactory epithelium	Present		3A

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present		3B
TS24: lung epithelium	Strong		3B
TS24: axial skeleton	Present	Regionally restricted	3B	Expression is in vertebra.
TS24: skeletal muscle	Present		3B

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted		The authors state that this gene was differentially expressed in the adult brain, but did not provide details.