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Gene Expression Data
Assay Details
Assay
Reference: J:81302 Fleming TP, et al., Localisation of tight junction protein cingulin is temporally and spatially regulated during early mouse development. Development. 1993 Mar;117(3):1135-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2450133
Gene symbol: Cgn
Gene name: cingulin
Antibody: anti-Cingulin
Detection system: Secondary antibody
Assay notes: For whole mount specimens, the fixative was 0.5% formaldehyde for 20 minutes. For sectioned specimens, the fixative was 1% formaldehyde.
Results
Specimen 1A: postnatal adult (more )
Note: Egg was freed from cumulus cells and zona pellucida.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 1A , 1B Expression was detected in the cytoplasmic face of the oolemma in 100% of oocytes. In the cytocortex, the microvillous domain was stained, but staining was absent or poor in the smooth membrane domain overlying the metaphase II spindle.

Specimen 1D: postnatal adult (more )
Note: Egg was freed from cumulus cells and zona pellucida.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 1D , 1C Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.

Specimen 1F: postnatal adult (more )
Note: Egg was freed from cumulus cells and zona pellucida.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 1E , 1F Expression was detected in the oocyte cortex in 100% of the oocytes. Staining was not detected in most cumulus cells except within the corona radiata weakly at the site of contact with the oocyte.

Specimen Not Shown: embryonic day 0.5 (more )
Note: Fertilized egg.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted Expression was detected in the cytoplasmic face of the oolemma of 100% of the fertilized eggs.

Specimen Not Shown: embryonic day 1.0 (more )
Note: 2-cell embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted Expression was detected in the cytocortex either uniformly or preferentially on the outer surface of 69% of embryos.

Specimen 2A: embryonic day 2.0 (more )
Note: 4-cell embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted 2A Expression was detected in the cytocortex either uniformly or preferentially on the outer surface of 78% of embryos.

Specimen Not Shown: embryonic day 2.5 (more )
Note: Precompact 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 57% of the embryos but absent from the nascent apicolateral tight junctions site.

Specimen 2B: embryonic day 2.5 (more )
Note: Compact 8-cell embryo.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 2B Expression in the cytocortex was detected in the apical microvillous pole on the outer surface of each blastomere in 85% of the embryos but absent from the nascent apicolateral tight junctions site.

Specimen 2C: embryonic day 3.0 (more )
Note: 16-cell embryo.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 2C Expression in the cytocortex was in the apical microvillous pole in outer blastomeres of the trophectoderm lineage of 67% of embryos. Faint zonular or discontinuous staining was in the forming tight junction sites of each blastomere in 75% of embryos.

Specimen Not Shown: embryonic day 3.5 (more )
Note: Late morula embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted Expression was absent from the inner cell mass and the cytocortex, but was present in the tight junctions in the trophectoderm in 100% of embryos and in punctate cytoplasmic foci surrounding the trophectoderm nuclei in 70% of embryos.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/28/2022
MGI 6.20
The Jackson Laboratory