Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71241
Brown CB, et al., PlexinA2 and semaphorin signaling during cardiac neural crest development. Development. 2001 Aug;128(16):3071-80
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2154280
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Gene symbol:
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Plxna2
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Gene name:
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plexin A2
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Probe:
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PlexinA2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 11.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: outflow tract |
Present |
Regionally restricted |
2A
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Expression in two prongs of cells extending into the septating outflow tract rostral to the right ventricle.
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Specimen
3A:
embryonic day 12.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3E:
embryonic day 12.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: endocardial cushion |
Present |
Regionally restricted |
3E
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Expressing cells from a "saddle" within the outflow endocardial cushions.
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| TS20: trunk mesenchyme |
Present |
Regionally restricted |
3E
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Expression surrounding the aorta and pulmonary trunk.
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Specimen
3I:
embryonic day 12.5
(more )
Specimen
3I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: endocardial cushion |
Present |
Regionally restricted |
3I
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Expression is localized to two clusters of cells within the endocardial cushions.
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Specimen
6A:
embryonic day 12.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Regionally restricted |
6A
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Prongs of expressing cells migarting the aortic arches and populating the outflow tract.
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| TS20: trunk mesenchyme |
Present |
Regionally restricted |
6A
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Expression surrounding the aorta and pulmonary trunk.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Sema3ctm1.1Jra/Sema3ctm1.1Jra
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: heart |
Present |
Regionally restricted |
6B
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Prongs of expressing cells that are reduced compared to wild-type embryos.
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| TS20: trunk mesenchyme |
Present |
Regionally restricted |
6B
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Expression surrounding the aorta and pulmonary trunk is reduced compared to wild-type embryos.
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Specimen
7A:
embryonic day 12.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7C:
embryonic day 12.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7D:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7F:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp/Pax3Sp
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools