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Gene Expression Data
Assay Details
Assay
Reference: J:53564 Dong LQ, et al., Primary structure, tissue distribution, and expression of mouse phosphoinositide-dependent protein kinase-1, a protein kinase that phosphorylates and activates protein kinase Czeta. J Biol Chem. 1999 Mar 19;274(12):8117-22
Assay type: Western blot
MGI Accession ID: MGI:1347445
Gene symbol: Pdpk1
Gene name: 3-phosphoinositide dependent protein kinase 1
Antibody: anti-mPDK1 antibody
Detection system: Secondary antibody coupled to alkaline phosphatase
Results
Image: 3 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 180 kDa (a) 125 kDa (a) 63 kDa Genetic BackgroundMutant Allele(s)Sex
1 P m 3-6 TS28: testis Present Present Very strong Not Specified Male
2 P m 3-6 TS28: brain Present Present Very strong Not Specified Not Specified
3 P m 3-6 TS28: heart Absent Absent Very strong Not Specified Not Specified
4 P m 3-6 TS28: metanephros Present Present Strong Not Specified Not Specified
5 P m 3-6 TS28: spleen Present Present Very strong Not Specified Not Specified
6 P m 3-6 TS28: adipose tissue Present Absent Very strong Not Specified Not Specified
7 P m 3-6 TS28: liver Present Present Strong Not Specified Not Specified
8 P m 3-6 TS28: skeletal muscle Absent Absent Weak Not Specified Not Specified
Notes:
(a) Preincubation of the antibody with excess amounts of GST-mPDK1>CT< antigen eliminated these protein bands, suggesting that the immunoreactivity was specific.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/05/2021
MGI 6.17
The Jackson Laboratory