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Gene Expression Data
Assay Details
Assay
Reference: J:50869 Ko MSH, et al., Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome. Hum Mol Genet. 1998 Nov;7(12):1967-78
Assay type: RT-PCR
MGI Accession ID: MGI:1332018
Gene symbol: Ddx21
Gene name: DExD box helicase 21
Probe: D10Wsu42e pA,pB
Visualized with: Ethidium bromide
Assay notes: Equal amounts of cDNA derived from extraembryonic tissues and embryonic tissues were sequentially diluted in 10-fold steps. Controls: the abundance of cytoskeletal beta-actin cDNA within extraembryonic tissue was shown to be the same as that in embryonic tissue; RNA samples without reverse transcriptase treatment did not show any PCR products.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1x E7.5 TS11: embryo Present Not Specified; total RNA C57BL/6J Not Specified
10x E7.5 TS11: embryo Absent Not Specified; total RNA C57BL/6J Not Specified
100x E7.5 TS11: embryo Absent Not Specified; total RNA C57BL/6J Not Specified
1x E7.5 TS11: extraembryonic component Present Not Specified; total RNA C57BL/6J Not Specified
10x E7.5 TS11: extraembryonic component Present Not Specified; total RNA C57BL/6J Not Specified
100x E7.5 TS11: extraembryonic component Absent Not Specified; total RNA C57BL/6J Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory