Batf3tm1(flpo)Tasic
Targeted Allele Detail
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Symbol: |
Batf3tm1(flpo)Tasic |
Name: |
basic leucine zipper transcription factor, ATF-like 3; targeted mutation 1, Bosiljka Tasic |
MGI ID: |
MGI:6514359 |
Synonyms: |
Batf3tm1(flpo)Hze |
Gene: |
Batf3 Location: Chr1:190830611-190841140 bp, + strand Genetic Position: Chr1, 96.28 cM, cytoband H6
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Alliance: |
Batf3tm1(flpo)Tasic page
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Batf3tm1(flpo)Tasic expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting vector contained, from 5' to 3', a partial Batf3 sequence within exon 3 up to and including the endogenous stop codon, an internal ribosome entry site 2 sequence (IRES2; allows translation initiation in the middle of an mRNA sequence), a FlpO recombinase gene (a codon-optimized FLPe gene-modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence, an AttB site, a PGK /gb2 promoter-Neomycin resistance gene-PGK polyA cassette and an AttP site. Chimeric mice were bred with C57BL/6J mice.
(J:101977)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Batf3
(mouse)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
2 reference(s) |
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