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Endonuclease-mediated Allele Detail
Symbol: Sox2em2.1(Snrpn-EGFP)Jae
Name: SRY (sex determining region Y)-box 2; endonuclease-mediated mutation 2.1, Rudolf Jaenisch
MGI ID: MGI:6383982
Gene: Sox2  Location: Chr3:34650405-34652461 bp, + strand  Genetic Position: Chr3, 16.93 cM, cytoband A2-B
Strain of Origin:  CAST/Ei
Allele Type:    Endonuclease-mediated (Reporter)
Mutation:    Insertion
Mutation detailsCRISPR/cas9 endonuclease-mediated genome editing in is used target the super enhancer (SE) sequences ~100 kb distal of the SRY (sex determining region Y)-box 2 locus (Sox2) on chromosome 3, as well as Snrpn::eGFP::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter [including conserved elements between human and mouse and containing the endogenous imprinted DMR region], the enhanced green fluorescent protein (EGFP), a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES cells with Snrpn::eGFP::WPRE::BHGpA sequences inserted into the Sox2 SE on the CAST chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro cassette. (J:282716)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sox2 Mutation:  23 strains or lines available
Original:  J:282716 Song Y, et al., Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Sep 5;75(5):905-920.e6
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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