Sox2em2.1(Snrpn-EGFP)Jae
Endonuclease-mediated Allele Detail
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Symbol: |
Sox2em2.1(Snrpn-EGFP)Jae |
Name: |
SRY (sex determining region Y)-box 2; endonuclease-mediated mutation 2.1, Rudolf Jaenisch |
MGI ID: |
MGI:6383982 |
Synonyms: |
SOX2-CASTSE-RGM-eGFP, SOX2-SE-G |
Gene: |
Sox2 Location: Chr3:34704554-34706610 bp, + strand Genetic Position: Chr3, 16.93 cM, cytoband A2-B
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Alliance: |
Sox2em2.1(Snrpn-EGFP)Jae page
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Allele Type: |
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Endonuclease-mediated (Reporter) |
Mutation: |
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Insertion
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Mutation details: CRISPR/cas9 endonuclease-mediated genome editing in is used target the super enhancer (SE) sequences ~100 kb distal of the SRY (sex determining region Y)-box 2 locus (Sox2) on chromosome 3, as well as Snrpn::eGFP::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter [including conserved elements between human and mouse and containing the endogenous imprinted DMR region], the enhanced green fluorescent protein (EGFP), a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES cells with Snrpn::eGFP::WPRE::BHGpA sequences inserted into the Sox2 SE on the CAST chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro cassette.
(J:282716)
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Original: |
J:282716 Song Y, et al., Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Sep 5;75(5):905-920.e6 |
All: |
1 reference(s) |
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