Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Fancatm1c(EUCOMM)Wtsi
Name:	Fanconi anemia, complementation group A; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID:	MGI:6369426
Synonyms:	Fancafl
Gene:	Fanca  Location: Chr8:123995039-124045315 bp, - strand  Genetic Position: Chr8, 72.1 cM
Alliance:	Fancatm1c(EUCOMM)Wtsi page
IMPC:	Fanca gene page

Germline Transmission:	Earliest citation of germline transmission: J:253597
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion   Mutation details: The L1L2_Bact_P cassette was inserted at position 124042863 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 124043667. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:253597)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fanca Mutation:	81 strains or lines available

Original:	J:253597 Garaycoechea JI, et al., Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018 Jan 11;553(7687):171-177
All:	2 reference(s)