Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Mlycdtm1c(KOMP)Wtsi
Name:	malonyl-CoA decarboxylase; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID:	MGI:6363077
Gene:	Mlycd  Location: Chr8:120121617-120137841 bp, + strand  Genetic Position: Chr8, 67.64 cM
Alliance:	Mlycdtm1c(KOMP)Wtsi page
IMPC:	Mlycd gene page

Germline Transmission:	Earliest citation of germline transmission: J:277712
Parent Cell Line:	JM8.F6 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	KOMP-CSD

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion   Mutation details: The L1L2_Bact_P cassette was inserted at position 120127799 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 120128565. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:277712)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mlycd Mutation:	19 strains or lines available

Original:	J:277712 Fisher-Wellman KH, et al., Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics. Cell Rep. 2019 Feb 5;26(6):1557-1572.e8
All:	1 reference(s)