Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Pdx1tm1.1(dre)Gkg
Name:	pancreatic and duodenal homeobox 1; targeted mutation 1.1, George K Gittes
MGI ID:	MGI:6359751
Synonyms:	Pdx1-Dre
Gene:	Pdx1  Location: Chr5:147206907-147212658 bp, + strand  Genetic Position: Chr5, 86.84 cM
Alliance:	Pdx1tm1.1(dre)Gkg page

Germline Transmission:	Earliest citation of germline transmission: J:278248
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Pdx1tm1.1(dre)Gkg expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Pdx1 (MGI:102851)   Mutation details: The targeting construct was generated such that exon 2 of the mouse Pdx1 gene in a BAC clone RP23-59N19 was replaced with a modified exon 2 in which a 2A peptide sequence followed by the Dre sequence was inserted immediately before the stop codon of the Pdx1-coding sequence. The FRT-flanked neomycin selection cassette (neo) was placed at the beginning of the 3' untranslated sequence which was removed by crossing chimeric mice with Flpo mice. (J:278248)

Activity:	Tissue activity of this recombinase allele
Driver:	Pdx1 (mouse) Summary of all recombinase alleles driven by Pdx1. Comparative matrix view of recombinase activities Alliance mouse Pdx1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pdx1 Mutation:	35 strains or lines available

Original:	J:278248 Chen C, et al., Evidence of a developmental origin for beta-cell heterogeneity using a dual lineage-tracing technology. Development. 2019 Jun 27;146(13):dev164913
All:	1 reference(s)