Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Bace1tm1c(EUCOMM)Hmgu
Name:	beta-site APP cleaving enzyme 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
MGI ID:	MGI:6258426
Synonyms:	BACE1fl
Gene:	Bace1  Location: Chr9:45749878-45775694 bp, + strand  Genetic Position: Chr9, 25.22 cM
Alliance:	Bace1tm1c(EUCOMM)Hmgu page
IMPC:	Bace1 gene page

Germline Transmission:	Earliest citation of germline transmission: J:266958
Parent Cell Line:	JM8A1.N3 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion   Mutation details: The L1L2_Bact_P cassette was inserted at position 45763695 of Chromosome 9 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 45764551. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele, removing the lacZ and neo cassettes. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:266958)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Bace1 Mutation:	27 strains or lines available

Original:	J:266958 Ou-Yang MH, et al., Axonal organization defects in the hippocampus of adult conditional BACE1 knockout mice. Sci Transl Med. 2018 Sep 19;10(459)
All:	1 reference(s)