Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Mbd2tm1.1Joez
Name:	methyl-CpG binding domain protein 2; targeted mutation 1.1, Zhaolan Zhou
MGI ID:	MGI:5924954
Synonyms:	Mbd2Tavi
Gene:	Mbd2  Location: Chr18:70701260-70759202 bp, + strand  Genetic Position: Chr18, 44.54 cM
Alliance:	Mbd2tm1.1Joez page

Germline Transmission:	Earliest citation of germline transmission: J:240794
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129

Allele Type:    Targeted (Epitope tag) Mutation:    Insertion   Mutation details: A targeting construct was designed to insert a loxP-flanked neomycin (neo) resistance cassette upstream of exon 6 of the X-linked methyl-CpG binding domain protein 2 (Mbd2) gene. A 23 amino acid Tavi tag, allowing MBD2 protein to be biotinylated in the presence of E. coli biotin ligase, BirA, was introduced immediately upstream of Mbd2 stop codon. Breeding to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J transgenic mice (Stock No. 003724) removed the neo cassette. (J:240794)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Mbd2 Mutation:	21 strains or lines available

Original:	J:240794 Wood KH, et al., Tagging methyl-CpG-binding domain proteins reveals different spatiotemporal expression and supports distinct functions. Epigenomics. 2016 Apr;8(4):455-73
All:	1 reference(s)