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Prkag2tm1.1Geno
Targeted Allele Detail
Nomenclature
Symbol: Prkag2tm1.1Geno
Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit; targeted mutation 1.1, Genoway
MGI ID: MGI:5907189
Synonyms: R299Q gamma2 AMPK
Gene: Prkag2  Location: Chr5:25067742-25305640 bp, - strand  Genetic Position: Chr5, 11.93 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:235379
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S/SvEv
Mutation
description
Allele Type:    Targeted (Conditional ready, Constitutively active)
Mutations:    Insertion, Single point mutation
 
Mutation detailsA c.896G>A mutation was engineered in exon 7 changing arginine codon 299 to a glutamine codon (p.Arg299Gln). This mutation is the equivalent of the human R302Q mutation which causes increased unstimulated activity and is associated with a cardiac phenotype. An FRT site flanked neomycin resistance gene cassette was inserted into intron 6 and removed later through flp-mediated recombination. In addition, loxP sites were inserted within intron 6 upstream and within intron 7 downstream from the mutation. (J:235379, J:256296)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prkag2 Mutation:  37 strains or lines available
References
Original:  J:235379 Yavari A, et al., Chronic Activation of gamma2 AMPK Induces Obesity and Reduces beta Cell Function. Cell Metab. 2016 May 10;23(5):821-36
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/17/2022
MGI 6.19
The Jackson Laboratory