Sox9em3Rlb
Endonuclease-mediated Allele Detail
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| Symbol: |
Sox9em3Rlb |
| Name: |
SRY (sex determining region Y)-box 9; endonuclease-mediated mutation 3, Robin Lovell-Badge |
| MGI ID: |
MGI:5824335 |
| Synonyms: |
TES- |
| Gene: |
Sox9 Location: Chr11:112673050-112678586 bp, + strand Genetic Position: Chr11, 77.27 cM
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| Alliance: |
Sox9em3Rlb page
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| Allele Type: |
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Endonuclease-mediated (Not Applicable) |
| Mutation: |
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Intergenic deletion
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Mutation details: The TES (testis specific enhancer of Sox9) regulatory region 10-13 kb upstream of the gene was targeted with a pair of single guide RNAs (sgRNAs) using the CRISPR/Cas9 system. The proximal sgRNA (5'-caccGGGAACATTGGCAGGGCCCT-3'; recognition sequence in capital letters) was targeted to sequence 20 bp downstream of the 3' end of the region. The distal sgRNA (caccGAGGTGTGTGGAAGCGGGC) was targeted to sequence 25 bp upstream of the 5' end of the region. Two stable lines were established that carried the 3194 bp deletion between the Cas9 cleavage sites. This 3194 bp deletion deletes the entire 3161 bp TES sequence.
(J:238708)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Sox9 Mutation: |
32 strains or lines available
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| Original: |
J:238708 Gonen N, et al., Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. PLoS Genet. 2017 Jan;13(1):e1006520 |
| All: |
1 reference(s) |
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Analysis Tools
