Sox9em1Rlb
Endonuclease-mediated Allele Detail
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| Symbol: |
Sox9em1Rlb |
| Name: |
SRY (sex determining region Y)-box 9; endonuclease-mediated mutation 1, Robin Lovell-Badge |
| MGI ID: |
MGI:5824333 |
| Synonyms: |
TESCO- |
| Gene: |
Sox9 Location: Chr11:112673050-112678586 bp, + strand Genetic Position: Chr11, 77.27 cM
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| Alliance: |
Sox9em1Rlb page
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| Allele Type: |
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Endonuclease-mediated (Not Applicable) |
| Mutation: |
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Intergenic deletion
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Mutation details: The core region (TESCO) of the TES regulatory region 10-13 kb upstream of the gene was targeted with a pair of single guide RNAs (sgRNAs) using the CRISPR/Cas9 system. The proximal sgRNA (5'-caccgTCTGTTAACGTATGACACGA-3'; recognition sequence in capital letters) was targeted to sequence 8 bp downstream of the 3'. The distal sgRNA (caccGTTGGAGTTCCGATTTAGA) was targeted to sequence 41 bp downstream of the 5' end of the region (i.e. inside the region). Two stable lines were established that carried the exact 1260 bp deletion between the Cas9 cleavage sites. This 1260 bp deletion deletes 1252 of the 1293 bp TESCO sequence. One line also carried an 11 bp insertion of unrecognized sequence at the cleavage site. Only one of the lines was used for the experiments.
(J:238708)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Sox9 Mutation: |
32 strains or lines available
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| Original: |
J:238708 Gonen N, et al., Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone. PLoS Genet. 2017 Jan;13(1):e1006520 |
| All: |
1 reference(s) |
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Analysis Tools
