Tg(Sox2-Bgeo)1Skn
Transgene Detail
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| Symbol: |
Tg(Sox2-Bgeo)1Skn |
| Name: |
transgene insertion 1, Silvia K Nicolis |
| MGI ID: |
MGI:5700640 |
| Synonyms: |
5.7 kb Sox2 transgene, Sox2 beta-geo telencephalic transgene |
| Transgene: |
Tg(Sox2-Bgeo)1Skn Location: unknown
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| Alliance: |
Tg(Sox2-Bgeo)1Skn page
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| Strain of Origin: |
C57BL/6 or DBA/2
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| Transgene Type: |
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Transgenic (Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Mutation details: Expression of the E. coli beta-galactosidase/neomycin phosphotransferase (betageo) fusion gene is driven by regulatory elements of the mouse Sox2 gene that are present within 5.7 kb upstream of the unique Not1 site in the 5' untranslated region (5'-UTR). Beta-galactosidase expression (visualized by X-gal staining) is confined almost entirely to the dorsal telencephalon in 12.5-d.p.c. transgenic embryos and becomes progressively more restricted as cortical development proceeds. Neural stem cells isolated from the ventricular zone of the telencephalon of 14.5-d.p.c. transgenic embryos stain with X-gal and are resistant to G418. In the adult brain, beta-galactosidase expression is observed in the periventricular cells of the lateral ventricle and in the rostral migratory stream reaching the olfactory bulb.
(J:62026)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Transgenic founder mice were generated by pronuclear microinjection of (C57BL/6 x DBA/2)F2 zygotes, derived from crosses between superovulated (C57BL/6 x DBA/2)F1 (B6D2F1) females and B6D2F1 males.
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| Original: |
J:62026 Zappone MV, et al., Sox2 regulatory sequences direct expression of a (beta)-geo transgene to telencephalic neural stem cells and precursors of the mouse embryo, revealing regionalization of gene expression in CNS stem cells. Development. 2000 Jun;127(11):2367-82 |
| All: |
1 reference(s) |
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Analysis Tools
