Rsph9tm1a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Rsph9tm1a(EUCOMM)Hmgu |
Name: |
radial spoke head 9 homolog (Chlamydomonas); targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5520238 |
Gene: |
Rsph9 Location: Chr17:46432961-46455142 bp, - strand Genetic Position: Chr17, 22.87 cM, cytoband C
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IMPC: |
Rsph9 gene page |
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Mutant Cell Lines: |
HEPD0916_10_A12, HEPD0916_10_B03, HEPD0916_10_B06, HEPD0916_10_B08, HEPD0916_10_B11, HEPD0916_10_B12, HEPD0916_10_C01, HEPD0916_10_C05, HEPD0916_10_C06, HEPD0916_10_C11, HEPD0916_10_D01, HEPD0916_10_D02, HEPD0916_10_D05, HEPD0916_10_D10, HEPD0916_10_E08, HEPD0916_10_F04, HEPD0916_10_F08, HEPD0916_10_F09, HEPD0916_10_G06, HEPD0916_10_H07, HEPD0916_10_H09, HEPD0916_10_H11 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 46452166 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 46453128. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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