Slc35e2tm2a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Slc35e2tm2a(EUCOMM)Hmgu |
Name: |
solute carrier family 35, member E2; targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5511706 |
Gene: |
Slc35e2 Location: Chr4:155685873-155707797 bp, + strand Genetic Position: Chr4, 86.62 cM
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Alliance: |
Slc35e2tm2a(EUCOMM)Hmgu page
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IMPC: |
Slc35e2 gene page |
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Mutant Cell Lines: |
HEPD0783_3_A05, HEPD0783_3_A06, HEPD0783_3_A07, HEPD0783_3_A08, HEPD0783_3_B05, HEPD0783_3_B08, HEPD0783_3_C07, HEPD0783_3_C08, HEPD0783_3_D08, HEPD0783_3_E05, HEPD0783_3_E06, HEPD0783_3_E07, HEPD0783_3_F05, HEPD0783_3_F08, HEPD0783_3_G05, HEPD0783_3_G07, HEPD0783_3_G08, HEPD0783_3_H05, HEPD0783_3_H06, HEPD0783_3_H07 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
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Parent Cell Line: |
JM8A3.N1.C2 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 155696611 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 155697414. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
2 reference(s) |
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