Myf5tm1.1(cre/Esr1*)Trdo
Targeted Allele Detail
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Symbol: |
Myf5tm1.1(cre/Esr1*)Trdo |
Name: |
myogenic factor 5; targeted mutation 1.1, Thomas A Rando |
MGI ID: |
MGI:5509340 |
Synonyms: |
Myf5CreER-NN |
Gene: |
Myf5 Location: Chr10:107318769-107321995 bp, - strand Genetic Position: Chr10, 55.95 cM
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Alliance: |
Myf5tm1.1(cre/Esr1*)Trdo page
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutations: |
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Insertion, Intragenic deletion
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Myf5tm1.1(cre/Esr1*)Trdo expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Myf5 (MGI:97252) |
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Mutation details: A targeting vector was designed to place an internal ribosome entry site (IRES) fused to a CreERTM fusion gene (CreERTM; Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand binding domain) downstream of the stop codon in exon 3 of the Myf5 gene. A neomycin resistance marker, flanked by frt sites, was inserted downstream of the IRES-CreERTM
cassette. This construct was electroporated into R1 embryonic stem cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric males were bred with C57BL/6 females. To remove the neo marker, mice were bred to B6.129S4-Gt(ROSA)26Sor/RainJ mice.
(J:199482)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Myf5
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Myf5 Mutation: |
17 strains or lines available
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Original: |
J:199482 Biressi S, et al., Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells. Dev Biol. 2013 Jul 15;379(2):195-207 |
All: |
3 reference(s) |
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