Nprl3tm2.1Drhi
Targeted Allele Detail
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Symbol: |
Nprl3tm2.1Drhi |
Name: |
nitrogen permease regulator-like 3; targeted mutation 2.1, Douglas R Higgs |
MGI ID: |
MGI:5426856 |
Synonyms: |
deltaP6-R3- |
Gene: |
Nprl3 Location: Chr11:32181963-32217707 bp, - strand Genetic Position: Chr11, 18.83 cM
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Alliance: |
Nprl3tm2.1Drhi page
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Germline Transmission: |
Earliest citation of germline transmission:
J:184965
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Parent Cell Line: |
E14TG2a (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Hypomorph, Modified regulatory region) |
Mutations: |
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Insertion, Intergenic deletion, Intragenic deletion
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Mutation details: In ES cells carrying the Nprl3 allele, Hba erythroid-specific enhancer R3 in Nprl3 intron 2 was replaced with a loxP site flanked neomycin resistance gene cassette. Subsequent cre-mediated recombination removed the neo cassette and the 12402 bp span between the loxP site in place of Nprl3 promoter P6 (Rr246493) and enhancer R3 (Rr351), which also serves as a promoter for Nprl3 transcripts with alternative, more downstream, 1st exons. The deletion also includes putative Hba enhancer Rm or DHS-12 (Rr352) in Nprl3 intron 1 and Nprl3 exons 1 and 2. RNA-FISH analysis indicates Nprl3 expression is eliminated in the brain but not in erythroid cells. Expression in erythroid cells from homozygous mice is about 20% that of wild-type, presumably from alternatively spliced transcripts with alternative 1st exons. No protein expression was detected in any tissue.
(J:184965)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Nprl3 Mutation: |
31 strains or lines available
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Original: |
J:184965 Kowalczyk MS, et al., Intragenic enhancers act as alternative promoters. Mol Cell. 2012 Feb 24;45(4):447-58 |
All: |
2 reference(s) |
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