Gt(ROSA)26Sortm1.1(CAG-mTFP1)Imayo
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1.1(CAG-mTFP1)Imayo |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Itaru Imayoshi |
MGI ID: |
MGI:5317933 |
Synonyms: |
R26-CAG-LoxP-mTFP1 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1.1(CAG-mTFP1)Imayo page
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Germline Transmission: |
Earliest citation of germline transmission:
J:182745
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Parent Cell Line: |
TC1/TC-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutation: |
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Insertion
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Mutation details: The vector R26-CAG-LF-mTFP1 was inserted into the locus. This vector contains (5' to 3') a splice acceptor, puro cassette, polyadenylation sequence, CAG promoter, floxed STOP cassette, FRT-flanked STOP cassette, HA-tagged mTFP1 (monomeric cyan fluorescent protein derived from the Clavularia fluorescent protein cFP484), and woodchuck hepatitis virus (WPRE) polyadenylation sequence. Flp-mediated recombination removed one of the two STOP cassette. The loxP-flanked stop cassette remains intact.
(J:182745)
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Original: |
J:182745 Imayoshi I, et al., A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination. Neurosci Res. 2012 May;73(1):85-91 |
All: |
10 reference(s) |
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