Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Liqun Luo |
MGI ID: |
MGI:5314254 |
Synonyms: |
R26G-tTA2 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo page
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Allele Type: |
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Targeted (Reporter, Transactivator) |
Mutation: |
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Insertion
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Mutation details: A targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the MADM G-tTA2 cassette. The MADM G-tTA2 cassette used here (GFPN-terminus-intron-tTA2ATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), an ATG-less tetracycline-regulated transactivator sequence (tTA2ATG-less), and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-G-tTA2. The pROSA26-G-tTA2 construct was inserted into the Gt(ROSA)26Sor locus.
(J:181830)
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Generation of various Gt(ROSA)26Sor alleles |
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Original: |
J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332 |
All: |
1 reference(s) |
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