Hprt1tm46(Ple112-lacZ)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm46(Ple112-lacZ)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 46, Elizabeth M Simpson |
MGI ID: |
MGI:5303424 |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Allele Type: |
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Targeted |
Mutation: |
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Insertion
Vector Type: Not Applicable
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Mutation details: The Ple112-lacZ plasmid (pEMS1583) was created by inserting the Ple112 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human hypocretin (orexin) neuropeptide precursor (HCRT) gene) into the multiple cloning site of the pEMS1313 plasmid vector backbone. This placed the Ple112 MiniPromoter upstream of an intron, a full F5 mutant-frt site, a Kozak consensus sequence, a beta-galactosidase (lacZ) gene, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple112-lacZ construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells.
(J:164356)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Hprt1 Mutation: |
1274 strains or lines available
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Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
All: |
2 reference(s) |
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