Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Hprt1tm326(Ple231-EGFP)Ems
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 326, Elizabeth M Simpson
MGI ID:	MGI:5303389
Gene:	Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutant Cell Line:	mEMS3231
Germline Transmission:	Unknown
Parent Cell Line:	mEMS1204 (ES Cell)
Strain of Origin:	(B6.129P2-Hprt1b-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection:	Pleiades Promoter Project

Allele Type:    Targeted (Reporter) Mutation:    Insertion     Vector Type: Not Applicable   Mutation details: The Ple231-EGFP plasmid (pEMS1435) was created by inserting the Ple231 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human troponin T type 1 (skeletal, slow) (TNNT1) gene) into the multiple cloning site of the pEMS1306 plasmid vector backbone. This placed the Ple231 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an enhanced green fluorescent protein (EGFP; with mutated TAA->TTA stop), a tetracysteine residue, a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple231-EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1204 embryonic stem cells: a male ES cell line from a B6129F1-Gt(ROSA)26Sortm1Sor/+ Hprtb-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). The resulting ES cells, correctly targeted with the Ple231-EGFP transgene inserted into the Hprt locus, were assigned Hprttm293(Ple231-EGFP)Ems. (J:164356)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1273 strains or lines available

Original:	J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:	1 reference(s)