Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Hprt1tm325(Ple157-cre/EGFP)Ems
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 325, Elizabeth M Simpson
MGI ID:	MGI:5303373
Gene:	Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutant Cell Line:	mEMS569
Germline Transmission:	Unknown
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd
Project Collection:	Pleiades Promoter Project

Allele Type:    Targeted (Recombinase, Reporter) Mutation:    Insertion     Vector Type: Not Applicable   Hprt1tm325(Ple157-cre/EGFP)Ems expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Homolog in Mouse Note human PCP2 (126006) Pcp2  (MGI:97508)   Mutation details: The Ple157-Cre/EGFP plasmid (pEMS1064) was created by inserting the Ple157 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human Purkinje cell protein 2 (PCP2) gene) into the multiple cloning site of the pEMS1040 plasmid vector backbone. This placed the Ple157 MiniPromoter upstream of a minimal wildtype frt site, a Cre/EGFP fusion gene (Cre recombinase gene and enhanced green fluorescent protein (EGFP; with mutated TAA->TTA stop)), a nuclear localization signal, an SV40 early mRNA polyA signal, a modified minimal frt site (A deletion), a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple157-Cre/EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells: a male ES cell line harboring the Hprtb-m3 mutation on the X Chromosome. The resulting ES cells, correctly targeted with the Ple157-Cre/EGFP transgene inserted into the Hprt locus, were assigned Hprttm28(Ple157-cre/EGFP)Ems. (J:164356)

Activity:	Tissue activity of this recombinase allele
Driver:	PCP2 (human) Summary of all recombinase alleles driven by PCP2. Alliance human PCP2 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1274 strains or lines available

Original:	J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:	1 reference(s)