Hprt1tm191(Ple142-EGFP)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm191(Ple142-EGFP)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 191, Elizabeth M Simpson |
MGI ID: |
MGI:5303274 |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Allele Type: |
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Targeted (Reporter) |
Mutation: |
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Insertion
Vector Type: Not Applicable
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Mutation details: The RP11-144P8 bacterial artificial chromosome (BAC) was obtained and retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector backbone. Next, an enhanced green fluorescent protein (EGFP) and frt-flanked kanamycin cassette were inserted directly at the ATG of the human nuclear receptor subfamily 2, group E, member 1 (NR2E1) gene; this created the Ple142-EGFP BAC (bEMS82, NR2E1). The kanamycin cassette was transiently removed via FLP recombination; leaving the EGFP gene followed by a single frt site. The final modified BAC was used as the source for the Ple142-EGFP transgene. The Ple142-EGFP transgene was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1204 embryonic stem cells.
(J:164356)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Hprt1 Mutation: |
1274 strains or lines available
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Original: |
J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94 |
All: |
2 reference(s) |
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