Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Hprt1tm185(Ple133-lacZ)Ems
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 185, Elizabeth M Simpson
MGI ID:	MGI:5303268
Gene:	Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6

Mutant Cell Lines:	mEMS4533, mEMS4551, mEMS4583, mEMS4584
Germline Transmission:	Unknown
Parent Cell Line:	mEMS1202 (ES Cell)
Strain of Origin:	(B6.129P2-Hprt1b-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection:	Pleiades Promoter Project

Allele Type:    Targeted Mutation:    Insertion     Vector Type: Not Applicable   Mutation details: The RP11-158L10 bacterial artificial chromosome (BAC) was obtained and retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector backbone. Next, a beta-galactosidase (lacZ) and frt-flanked kanamycin cassette were inserted directly at the ATG of the human nerve growth factor receptor (NGFR) gene; this created the Ple133-lacZ BAC (bEMS88, NGFR). The kanamycin cassette was transiently removed via FLP recombination; leaving the lacZ gene followed by a single frt site. The final modified BAC was used as the source for the Ple133-lacZ transgene. The Ple133-lacZ transgene was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. (J:164356)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1273 strains or lines available

Original:	J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:	1 reference(s)