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Hprt1tm321(Ple61-EGFP)Ems
Targeted Allele Detail
Summary
Symbol: Hprt1tm321(Ple61-EGFP)Ems
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 321, Elizabeth M Simpson
MGI ID: MGI:5303204
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Mutation
origin
Mutant Cell Lines:  mEMS2197, mEMS2830
Germline Transmission:  Unknown
Parent Cell Line:  mEMS1218 (ES Cell)
Strain of Origin:  (B6.129P2-Hprt1b-m3/J x 129S-Gt(ROSA)26Sortm1Sor/J)F1
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
 
Mutation detailsThe Ple61-EGFP plasmid (pEMS1166) was created by inserting the Ple61 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human dopamine receptor D1 (DRD1) gene) into the multiple cloning site of the pEMS1306 plasmid vector backbone. This placed the Ple61 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an enhanced green fluorescent protein (EGFP; with mutated TAA->TTA stop), a tetracysteine residue, a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple61-EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1218 embryonic stem cells: a male ES cell line from a B6129F1-Gt(ROSA)26Sortm1Sor/+ Hprtb-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for a Hprtb-m3 mutation [originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 002171 and 003310, respectively)). The resulting ES cells, correctly targeted with the Ple61-EGFP transgene inserted into the Hprt locus, were assigned Hprttm121(Ple61-EGFP)Ems. (J:164356)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1274 strains or lines available
References
Original:  J:164356 Portales-Casamar E, et al., A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory