Faf2TgTn(sb-Tyr)1799AOve
Transgenic Allele Detail
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Symbol: |
Faf2TgTn(sb-Tyr)1799AOve |
Name: |
Fas associated factor family member 2; transgenic transposon concatemer 1799A, Paul A Overbeek |
MGI ID: |
MGI:5300387 |
Gene: |
Faf2 Location: Chr13:54769597-54811876 bp, + strand Genetic Position: Chr13, 28.88 cM
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Alliance: |
Faf2TgTn(sb-Tyr)1799AOve page
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Transgene Type: |
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Transgenic (Transposon concatemer) |
Mutation: |
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Transposon insertion
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Mutation details: A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-Tyro-Gal4-SV40 transposon vector used here has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), a Gal4-VP16-SV40pA sequence (1.7 kbp), the Tyro minigene (4.1 kbp), and right IR/DR (280 bp). The IR/DR sequences are outward-facing (pointed away from the Gal4-VP16-SV40pA::Tyro sequences). The Gal4-VP16-SV40 cassette is in the opposite orientation to the Tyro minigene. The 4.1 kb tyrosinase minigene (Tyro) was isolated from plasmid Ty811C and contains the tyrosinase upstream regulatory sequences (2.25 kb from the BALB/c tyrosinase promoter and the first 65 bp of exon 1) and a chimeric cDNA made with (from 5' to 3') the upstream part of the C57BL/6-derived Tyrs-J (cysteine at amino acid 103) sequence ligated at a central ScaI site to the downstream part of the DBA/2-derived tyrosinase (valine at amino acid 346). Line 1799A contains 15 copies of the transposon on chromosome 13.
(J:178818)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Faf2 Mutation: |
24 strains or lines available
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Original: |
J:178818 Wang B, et al., Transposon mutagenesis with coat color genotyping identifies an essential role for Skor2 in sonic hedgehog signaling and cerebellum development. Development. 2011 Oct;138(20):4487-97 |
All: |
1 reference(s) |
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