Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Lefty2tm2Hmd
Name:	left-right determination factor 2; targeted mutation 2, Hiroshi Hamada
MGI ID:	MGI:2183818
Synonyms:	lefty2deltaASE, Rr179tm2Hmd
Gene:	Lefty2  Location: Chr1:180720673-180726668 bp, + strand  Genetic Position: Chr1, 84.46 cM
Alliance:	Lefty2tm2Hmd page

Germline Transmission:	Earliest citation of germline transmission: J:70483
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Modified regulatory region, Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A 0.5 kb region of the gene containing the asymmetric enhancer (ASE) was replaced with a loxP site flanked neomycin resistance gene cassette via homologous recombination. The floxed neo cassette was subsequently removed by crossing chimeric animals to a cre-expressing line. Ablation of asymmetric gene expression in homozygous mutant embryos was confirmed by whole mount in situ hybridization. (J:70483)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

8 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lefty2 Mutation:	14 strains or lines available

Original:	J:70483 Meno C, et al., Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. Dev Cell. 2001 Jul;1(1):127-138
All:	2 reference(s)