Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Jeremy Nathans
MGI ID:	MGI:3849063
Synonyms:	R26rtTACreER
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat page

Germline Transmission:	Earliest citation of germline transmission: J:155417
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129

Allele Type:    Targeted (Inducible, Recombinase, Transactivator) Inducer:    tamoxifen and tetracycline Mutation:    Insertion   Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note bacteria tetO   Mutation details: The construct consists of the rtTA coding region preceded by a splice acceptor so that it can be spliced onto the 5' exon (exon1) of the endogenous ROSA26 transcript, and is followed by six repeats of an SV40 transcriptional terminator signal ("stop"). In the presence of doxycycline, the rtTA protein activates transcription from the seven head-to-tail Tet response elements immediately 5' of a minimal cytomegalovirus immediate early promoter (TRE) of the CreER fusion protein open reading frame (followed by the bovine growth hormone 3' UTR and transcriptional terminator region), leading to expression of CreER. (J:155417)

Generation of the Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat allele

Activity:	Tissue activity of this recombinase allele
Driver:	tetO (bacteria) Summary of all recombinase alleles driven by tetO.

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:155417 Badea TC, et al., New mouse lines for the analysis of neuronal morphology using CreER(T)/loxP-directed sparse labeling. PLoS One. 2009;4(11):e7859
All:	4 reference(s)