Nrastm1e(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Nrastm1e(EUCOMM)Hmgu |
Name: |
neuroblastoma ras oncogene; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5002954 |
Gene: |
Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
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IMPC: |
Nras gene page |
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Mutant Cell Lines: |
HEPD0719_5_A02, HEPD0719_5_A03, HEPD0719_5_A04, HEPD0719_5_B02, HEPD0719_5_B04, HEPD0719_5_C03, HEPD0719_5_D02, HEPD0719_5_D04, HEPD0719_5_E01, HEPD0719_5_E03, HEPD0719_5_E04, HEPD0719_5_F01, HEPD0719_5_F03, HEPD0719_5_G03, HEPD0719_5_G04, HEPD0719_5_H01, HEPD0719_5_H03, HEPD0719_5_H04, HEPD0739_2_D02, HEPD0739_2_G04, HEPD0739_2_H03 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 102967001 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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