Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Siaetm1.1Avrk
Name:	sialic acid acetylesterase; targeted mutation 1.1, Ajit Varki
MGI ID:	MGI:3830709
Synonyms:	Siaeflox
Gene:	Siae  Location: Chr9:37525117-37559554 bp, + strand  Genetic Position: Chr9, 20.79 cM
Alliance:	Siaetm1.1Avrk page

Germline Transmission:	Earliest citation of germline transmission: J:144035
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Conditional ready, No functional change) Mutations:    Insertion, Intragenic deletion   Mutation details: This locus can generate two alternatively spliced variants that encode either a lysosomal sialic acid acetylesterase (Lse) or a cytosolic esterase (Cse). Exon 2 is unique to the Lse transcript, encoding the N-terminus of the protein product. A floxed neomycin resistance cassette was placed upstream of exon 2 and an additional loxP site was placed downstream of exon 2. Transient cre expression in correctly targeted ES cells removed the neomycin selection cassette leaving exon 2 flanked by loxP sites. (J:144035)

Generation of the Siaetm1.1Avrk and Siaetm1.2Avrk alleles

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Siae Mutation:	33 strains or lines available

Original:	J:144035 Cariappa A, et al., B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase. J Exp Med. 2009 Jan 16;206(1):125-38
All:	1 reference(s)