Tg(CTSG-NUP98/HOXA9)1589Tanak
Transgene Detail
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| Symbol: |
Tg(CTSG-NUP98/HOXA9)1589Tanak |
| Name: |
transgene insertion 1589, Takuro Nakamura |
| MGI ID: |
MGI:3822555 |
| Synonyms: |
NUP98-HOXA9 transgene |
| Transgene: |
Tg(CTSG-NUP98/HOXA9)1589Tanak Location: unknown
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| Alliance: |
Tg(CTSG-NUP98/HOXA9)1589Tanak page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(CTSG-NUP98/HOXA9)1589Tanak expresses
2 genes
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
HOXA9 (3205) |
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Transgene codes for a fusion protein of NUP98 and HOXA9. |
| human |
NUP98 (4928) |
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Transgene codes for a fusion protein of NUP98 and HOXA9. |
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Mutation details: The protein-coding sequence of this transgene was amplified from a fusion transcript that is expressed in patients with myeloid leukemias characterized by the t(7;11)(p15;p15) chromosomal translocation. The transcript, derived from the in-frame joining of the 141 bp 5' exon of NUP98 to exon 1B of HOXA9, generates a chimeric protein comprising the amino terminal half of NUP98, containing the phenylalanine-glycine (FG) repeat region, followed by most of HOXA9 including its homeodomain. The cDNA was inserted at the transcription start site of the human cathepsin G (CTSG) gene in an expression cassette containing the entire CTSG genomic sequence, including ~2.5 kb of 5' and ~0.8 kb of 3' flanking DNA, which drives expression exclusively in cells of the myeloid lineage. RT-PCR analysis detects transcripts derived from the transgene only in bone marrow and spleen.
(J:47502, J:96544, J:142951)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Of five transgenic founder lines, two - designated 131 and 1589 - were maintained. The latter was chosen for detailed phenotypic characterization because of its higher incidence of leukemia (10% vs. 7% at 12 months of age).
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| Original: |
J:96544 Iwasaki M, et al., Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model. Blood. 2005 Jan 15;105(2):784-93 |
| All: |
4 reference(s) |
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Analysis Tools
