Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 34.1, Hongkui Zeng
MGI ID:	MGI:4947243
Synonyms:	Ai34, R26synaptophysin-tdTomato, Rosa-CAG-LSL-Syp-tdTomato-deltaNeo
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm34.1(CAG-Syp/tdTomato)Hze page

Germline Transmission:	Earliest citation of germline transmission: J:170755
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Reporter) Mutation:    Insertion   Mutation details: The Rosa-CAG-LSL-Synaptophysin-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a Synaptophysin-tdTomato fusion protein sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. To create the Synaptophysin-tdTomato fusion protein sequence, a mouse synaptophysin cDNA sequence (encoding a 308 amino acid transcript with all but the first six amino acids of the full-length protein) was fused in-frame via a six nucleotide linker to the amino terminus of the tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). PhiC31-mediated recombination removed the neo cassette. (J:170755)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	944 strains or lines available

Original:	J:170755 Zeng H, Direct Data Submission 2011/04/15. MGI Direct Data Submission. 2011;
All:	47 reference(s)