Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Ttrtm2.1(TTR)Kymm
Name:	transthyretin; targeted mutation 2.1, Ken-ichi Yamamura
MGI ID:	MGI:4946280
Synonyms:	I(-)P(-)
Gene:	Ttr  Location: Chr18:20798337-20807378 bp, + strand  Genetic Position: Chr18, 11.47 cM
Alliance:	Ttrtm2.1(TTR)Kymm page

Germline Transmission:	Earliest citation of germline transmission: J:170043
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Inserted expressed sequence, RMCE-ready) Mutations:    Insertion, Intragenic deletion   Mutation details: ES cells containing the Ttrtm1Kymm null allele that serves as acceptor in recombination mediated cassette exchange were electroporated with a replacement vector and a cre recombinase expression vector. The replacement vector consisted of a wild-type human TTR cDNA and a FRT-flanked puromycin resistance gene flanked by a lox66 and a loxP site. Flp-mediated recombination removed the puro cassette. (J:170043)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ttr Mutation:	44 strains or lines available

Original:	J:170043 Li Z, et al., Enhanced expression of human cDNA by phosphoglycerate kinase promoter-puromycin cassette in the mouse transthyretin locus. Transgenic Res. 2011 Feb;20(1):191-200
All:	1 reference(s)