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Esr1tm1(NCOM)Mfgc
Targeted Allele Detail
Nomenclature
Symbol: Esr1tm1(NCOM)Mfgc
Name: estrogen receptor 1 (alpha); targeted mutation 1, Mammalian Functional Genomics Centre
MGI ID: MGI:4880399
Gene: Esr1  Location: Chr10:4611989-5005633 bp, + strand  Genetic Position: Chr10, 2.03 cM
Mutation
origin
Mutant Cell Lines:  N01838P1_C_169W_C4, N01838P1_C_169W_D2, N01838P1_C_169W_G1, N01838P1_C_169W_H1, N01838P1_C_169W_H2
Germline Transmission:  Unknown
Parent Cell Line:  C2 (Nagy) (ES Cell)
Strain of Origin:  C57BL/6NCrl
Project Collection: NorCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_GOHANU
 
Mutation detailsThe insertion of NorCOMM cassette pGOHANU created a deletion of size 966bp between positions 5600446-5599480 of Chromosome 10 (Genome Build NCBIM37). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Esr1 Mutation:  13 strains or lines available
References
Original:  J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010;
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
03/13/2019
MGI 6.13
The Jackson Laboratory