F8tm1(NCOM)Cmhd
Targeted Allele Detail
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| Symbol: |
F8tm1(NCOM)Cmhd |
| Name: |
coagulation factor VIII; targeted mutation 1, Centre for Modeling Human Disease |
| MGI ID: |
MGI:4880047 |
| Gene: |
F8 Location: ChrX:74216321-74426221 bp, - strand Genetic Position: ChrX, 38.17 cM, cytoband A7-B
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| IMPC: |
F8 gene page |
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| Mutant Cell Line: |
N00132P0_C_101T_G11 |
| Germline Transmission: |
Unknown
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| Parent Cell Line: |
C2 (Tcp) (ES Cell)
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| Strain of Origin: |
C57BL/6N
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| Project Collection: |
NorCOMM
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_NTARU-1
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Mutation details: The insertion of NorCOMM cassette pNTARU created a deletion of size 4163bp between positions 74374734-74378897 of Chromosome X (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78).
(J:165963)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any F8 Mutation: |
24 strains or lines available
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| Original: |
J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010; |
| All: |
1 reference(s) |
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Analysis Tools
