Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Slc22a5tm1a(EUCOMM)Wtsi
Name:	solute carrier family 22 (organic cation transporter), member 5; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID:	MGI:4842338
Gene:	Slc22a5  Location: Chr11:53755368-53782486 bp, - strand  Genetic Position: Chr11, 32.02 cM
IMPC:	Slc22a5 gene page

Mutant Cell Lines:	EPD0215_4_A08, EPD0215_4_E08, EPD0215_4_F08, EPD0215_4_G07
Germline Transmission:	Unknown
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 53765423 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 53766410. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Slc22a5 Mutation:	29 strains or lines available

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	2 reference(s)