Summary | Mutation origin | Mutation description | Expression | Find Mice (IMSR) | Notes | References

Symbol:	Shank3tm1a(KOMP)Mbp
Name:	SH3 and multiple ankyrin repeat domains 3; targeted mutation 1a, Mouse Biology Program, UC Davis
MGI ID:	MGI:4454002
Gene:	Shank3  Location: Chr15:89383826-89444464 bp, + strand  Genetic Position: Chr15, 44.96 cM
Alliance:	Shank3tm1a(KOMP)Mbp page
IMPC:	Shank3 gene page

Mutant Cell Lines:	DEPD00516_3_A07, DEPD00516_3_B09, DEPD00516_3_B11, DEPD00516_3_B12, DEPD00516_3_C07, DEPD00516_3_C08, DEPD00516_3_C09, DEPD00516_3_C10, DEPD00516_3_C12, DEPD00516_3_D07, DEPD00516_3_D09, DEPD00516_3_E07, DEPD00516_3_E08, DEPD00516_3_E09, DEPD00516_3_E10, DEPD00516_3_E11, DEPD00516_3_E12, DEPD00516_3_F07, DEPD00516_3_F08, DEPD00516_3_F09, DEPD00516_3_F11, DEPD00516_3_F12, DEPD00516_3_G07, DEPD00516_3_H07, DEPD00516_3_H08, DEPD00516_3_H09, DEPD00516_3_H10
Germline Transmission:	Earliest citation of germline transmission: J:233405
Parent Cell Line:	JM8A1.N3 (ES Cell)
Strain of Origin:	C57BL/6N-Atm1Brd
Project Collection:	KOMP-CSD

Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Pgk_P   Mutation details: The L1L2_Pgk_P cassette was inserted at position 89389112 of Chromosome 15 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 89389949. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

In Mice Carrying this Mutation:

51 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Shank3 Mutation:	77 strains or lines available

Original:	J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010;
All:	2 reference(s)