Gt(ROSA)26Sortm1.1(CMV-luc,-ALPP)Cklr
Targeted Allele Detail
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| Symbol: |
Gt(ROSA)26Sortm1.1(CMV-luc,-ALPP)Cklr |
| Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Charles Keller |
| MGI ID: |
MGI:4437208 |
| Synonyms: |
LuSEAP, Rosa26LUSEAPm |
| Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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| Alliance: |
Gt(ROSA)26Sortm1.1(CMV-luc,-ALPP)Cklr page
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| Allele Type: |
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Targeted (Conditional ready, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A dual-modality bioreporter was targeted to the Rosa26 locus in intron 1. In addition to the native Rosa26 promoter, the CMV immediate-early promoter/enhancer and the SV40 late viral protein gene 16S/19S splice donor and acceptor (sd/sa) signal sites were added to strengthen reporter gene activity. To minimize leakage, a stop cassette consisting of 6 tandem copies of the SV40 viral early and late polyadenylation signal sequences [(pA)6] were inserted downstream of the promoter elements, flanked by LoxP sites. The stop cassette is followed by firefly luciferase, a human IRES, and human placental secreted alkaline phosphatase. An FRT-flanked neo selection followed the hPLAP gene and was removed by breeding to FLPe transgenic mice.
(J:157754)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Gt(ROSA)26Sor Mutation: |
942 strains or lines available
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| Original: |
J:157754 Nishijo K, et al., Biomarker system for studying muscle, stem cells, and cancer in vivo. FASEB J. 2009 Aug;23(8):2681-90 |
| All: |
5 reference(s) |
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Analysis Tools
