About   Help   FAQ
Nfasctm1a(EUCOMM)Hmgu
Targeted Allele Detail
Nomenclature
Symbol: Nfasctm1a(EUCOMM)Hmgu
Name: neurofascin; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4435919
Gene: Nfasc  Location: Chr1:132564690-132741797 bp, - strand  Genetic Position: Chr1, 57.42 cM
Mutation
origin
Mutant Cell Lines:  HEPD0529_1_B02, HEPD0529_1_B03, HEPD0529_1_C03, HEPD0529_1_D03, HEPD0529_1_E03, HEPD0529_1_E04, HEPD0529_1_F04, HEPD0529_1_G01, HEPD0529_1_G03, HEPD0529_1_G04, HEPD0529_1_H01, HEPD0529_1_H03, HEPD0529_1_H04
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 132635290 of Chromosome 1 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 132633942. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Nfasc Mutation:  8 strains or lines available
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
01/15/2019
MGI 6.13
The Jackson Laboratory