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Targeted Allele Detail
Symbol: Hcn4tm1a(EUCOMM)Hmgu
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4435096
Gene: Hcn4  Location: Chr9:58823512-58860955 bp, + strand  Genetic Position: Chr9, 31.8 cM, cytoband C
Mutant Cell Lines:  HEPD0547_4_A09, HEPD0547_4_A10, HEPD0547_4_B10, HEPD0547_4_B11, HEPD0547_4_B12, HEPD0547_4_C10, HEPD0547_4_C11, HEPD0547_4_D10, HEPD0547_4_D11, HEPD0547_4_E09, HEPD0547_4_E12, HEPD0547_4_F11, HEPD0547_4_F12, HEPD0547_4_G09, HEPD0547_4_G11, HEPD0547_4_H09, HEPD0547_4_H12
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 58843437 of Chromosome 9 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 58844550. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Hcn4 Mutation:  33 strains or lines available
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory