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Alg9tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Alg9tm1a(EUCOMM)Wtsi
Name: asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase); targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4433154
Gene: Alg9  Location: Chr9:50686525-50754939 bp, + strand  Genetic Position: Chr9, 27.75 cM
IMPC: Alg9 gene page
Mutation
origin
Mutant Cell Lines:  EPD0192_1_A09, EPD0192_1_A10, EPD0192_1_A12, EPD0192_1_B12, EPD0192_1_C12, EPD0192_1_D09, EPD0192_1_D11, EPD0192_1_E11, EPD0192_1_E12, EPD0192_1_F09, EPD0192_1_F10, EPD0192_1_F11, EPD0192_1_G12, EPD0192_1_H11
Germline Transmission:  Unknown
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 50694099 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 50694902. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Alg9 Mutation:  21 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/26/2022
MGI 6.22
The Jackson Laboratory