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Pik3c3tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Pik3c3tm1a(EUCOMM)Wtsi
Name: phosphatidylinositol 3-kinase catalytic subunit type 3; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4433098
Gene: Pik3c3  Location: Chr18:30272747-30348126 bp, + strand  Genetic Position: Chr18, 17.41 cM
Mutation
origin
Mutant Cell Lines:  EPD0103_4_A07, EPD0103_4_B07, EPD0103_4_B08, EPD0103_4_D07, EPD0103_4_D09, EPD0103_4_E08, EPD0103_4_F09, EPD0103_4_G08, EPD0103_4_G09, EPD0103_4_H09
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt2
 
Mutation detailsThe L1L2_gt2 cassette was inserted at position 30281495 of Chromosome 18 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30282336. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Generation of the Pik3c3tm1a(EUCOMM)Wtsi and Pik3c3tm1c(EUCOMM)Wtsi alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Pik3c3 Mutation:  32 strains or lines available
Notes
Cell line EPD0103_4_A07 was used to make chimeric mice. Germline transmission was accomplished. J:182628
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
01/15/2019
MGI 6.13
The Jackson Laboratory