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Drg2tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Drg2tm1a(EUCOMM)Wtsi
Name: developmentally regulated GTP binding protein 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432942
Gene: Drg2  Location: Chr11:60454617-60468752 bp, + strand  Genetic Position: Chr11, 37.81 cM
Mutation
origin
Mutant Cell Lines:  EPD0215_4_A10, EPD0215_4_A11, EPD0215_4_A12, EPD0215_4_B09, EPD0215_4_B10, EPD0215_4_B12, EPD0215_4_C10, EPD0215_4_C12, EPD0215_4_D12, EPD0215_4_E09, EPD0215_4_E10, EPD0215_4_E12, EPD0215_4_F09, EPD0215_4_F10, EPD0215_4_F11, EPD0215_4_F12, EPD0215_4_G09, EPD0215_4_G12
Germline Transmission:  Earliest citation of germline transmission: J:165965
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 60456306 of Chromosome 11 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 60456953. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Drg2 Mutation:  6 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
01/08/2019
MGI 6.13
The Jackson Laboratory