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Ldhatm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ldhatm1a(EUCOMM)Wtsi
Name: lactate dehydrogenase A; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4432392
Gene: Ldha  Location: Chr7:46841475-46855627 bp, + strand  Genetic Position: Chr7, 30.6 cM
Mutation
origin
Mutant Cell Lines:  EPD0070_2_A04, EPD0070_2_A05, EPD0070_2_A06, EPD0070_2_B04, EPD0070_2_C06, EPD0070_2_D05, EPD0070_2_E05, EPD0070_2_F04, EPD0070_2_F05, EPD0070_2_F06, EPD0070_2_G04, EPD0070_2_G05, EPD0070_2_G06, EPD0070_2_H04, EPD0070_2_H05, EPD0070_2_H06
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 46848508 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 46849394. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ldha Mutation:  23 strains or lines available
Notes
Cell line EPD0070_2_G04 was successfully used to make chimeric mice. Germline transmission was accomplished. J:214778
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/12/2018
MGI 6.12
The Jackson Laboratory