Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Ppp3catm2e(EUCOMM)Wtsi
Name:	protein phosphatase 3, catalytic subunit, alpha isoform; targeted mutation 2e, Wellcome Trust Sanger Institute
MGI ID:	MGI:4432193
Gene:	Ppp3ca  Location: Chr3:136375885-136643488 bp, + strand  Genetic Position: Chr3, 63.34 cM
Alliance:	Ppp3catm2e(EUCOMM)Wtsi page
IMPC:	Ppp3ca gene page

Mutant Cell Lines:	EPD0200_3_D03, EPD0200_3_E03, EPD0200_3_H01
Germline Transmission:	Earliest citation of germline transmission: J:204812
Parent Cell Line:	JM8.N4 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 136573894 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ppp3ca Mutation:	54 strains or lines available

Original:	J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:	5 reference(s)