Gt(ROSA)26Sortm1(CAG-EGFP)Fsh
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1(CAG-EGFP)Fsh |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Gordon Fishell |
MGI ID: |
MGI:4420759 |
Synonyms: |
RCE:dual |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1(CAG-EGFP)Fsh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:155164
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Parent Cell Line: |
W4 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Reporter) |
Mutation: |
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Insertion
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Mutation details: The R26R CAG-boosted EGFP (RCE) reporter targeting vector was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a splice acceptor site, a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA and 3x-polyA), an FRT-flanked modified pBS302 STOP cassette, an enhanced green fluorescent protein (EGFP) sequence, and two AttP-polyA sequences. After removal of the flanked STOP cassettes via cre- and Flp-mediated recombination, the EGFP reporter is expressed in cells/tissues where the expression patterns of the individual promoters driving Cre recombinase and FLP recombinase overlap.
(J:155164)
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Original: |
J:155164 Sousa VH, et al., Characterization of Nkx6-2-derived neocortical interneuron lineages. Cereb Cortex. 2009 Jul;19 Suppl 1:i1-10 |
All: |
20 reference(s) |
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